Thermo Fisher Scientific Phospho-Bora (Ser497) Polyclonal Antibody

Thermo Fisher Scientific Phospho-Bora (Ser497) Polyclonal Antibody

Applications

• Western Blot (WB)
  Tested Dilution: 1:1,000–1:3,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of BORA only when phosphorylated at Ser497.

Target Information

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), a centrosome-associated serine/threonine kinase regulating centrosome maturation, bipolar spindle assembly, and chromosome segregation during mitosis.
Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2-dependent manner. Activation of Cdc2 releases Bora into the cytoplasm, allowing it to bind and activate ARK-1.
PLK1 (polo-like kinase-1) interacts with Bora to control activation loop accessibility for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and regulate mitotic entry.
Bora mutants result in multipolar spindles similar to those seen when ARK-1 is inhibited, illustrating the ARK1-Bora-PLK1 regulatory circuit’s role in cell cycle control.
At least three isoforms of Bora are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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