Thermo Fisher Scientific FGF19 Monoclonal Antibody (117611)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

117611

Immunogen

E. coli-derived recombinant human FGF-19 Phe27-Lys216 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FGF19,uniProtId:O95750-1,ncbiNodeId:9606,antigenRange:27-216,antigenLength:216,antigenImageFileName:MA5-23861_FGF19_O95750-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23861_FGF19_O95750-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609888

Product Specific Information

This antibody shows approximately 10% cross-reactivity with recombinant human (rh) FGF-3 and rhFGF-5 and no cross-reactivity with rhFGF-4, rhFGF-6, rhFGF-7, rhFGF-9, rhFGF-10, rhFGF-13, rhFGF-16, rhFGF-17, rhFGF-18, rhFGF acidic, and rhFGF basic.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. The orthologous protein in mouse is FGF15, which shares about 50% amino acid identity and has similar functions. Together they are often referred to as FGF15/19.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.