Thermo Fisher Scientific Phospho-CDC25B (Ser323) Polyclonal Antibody
CDC25B 단백질의 Ser323 잔기 인산화 형태를 특이적으로 인식하는 rabbit polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 시료에서 반응하며, 1 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CDC25B (Accession P30305), corresponding to amino acid residues around phosphorylated Ser323. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816337 |
Product Specific Information
This antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 323.
Target Information
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in an ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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