Thermo Fisher Scientific TCRa/b Monoclonal Antibody (IP26)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive control: Tonsil
• Cellular location: Membrane

Target Information

The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression.
T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.

The majority of T cells in blood, lymph, and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas T cells expressing TCR gamma/delta heterodimers are mainly localized in epithelial tissues and infection sites.
The subunits of TCR heterodimers are covalently bonded and associate with CD3 subunits in the endoplasmic reticulum to form a functional TCR-CD3 complex. Lack of expression of any chain halts surface expression.

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, which is essential for effective adaptive immune responses. Recognition of self-peptides by TCRs is linked to autoimmune diseases, and mutant self-peptides have been associated with tumors.
Engagement of TCRs by bacterial superantigens can cause toxic shock syndrome. Autoantibodies to V beta segments of TCRs have been identified in patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), potentially modulating TH1-mediated inflammatory responses.

Most human T cells express TCR alpha-beta and either CD4 or CD8 (single positive, SP). A smaller subset lacks both CD4 and CD8 (double negative, DN), which may increase in autoimmune or immunodeficiency disorders. Gamma-delta T cells, primarily found in epithelial tissues, show less TCR diversity and distinct antigen recognition, with some subsets exhibiting antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.