Thermo Fisher Scientific G-CSF Polyclonal Antibody, PeproTech

Thermo Fisher Scientific G-CSF Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine G-CSF. Anti-Murine G-CSF-specific antibody was purified by affinity chromatography employing an immobilized Murine G-CSF matrix.

Sandwich ELISA:
To detect Murine G-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine G-CSF (500-P69Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine G-CSF.

Western Blot:
To detect Murine G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Murine G-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

G-CSF (Granulocyte colony stimulating factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described.
It is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells. G-CSF affects proliferation, differentiation, and activation of hematopoietic cells and is expressed in transformed cells such as carcinoma and myeloblastic leukemia cells.
Clinical applications include treatment of neonatal infections, acute myocardial infarction, neutropenia, severe infections, sepsis, autoimmune neutropenia, acute myeloid leukemia, Sweet’s syndrome, and AIDS. Dysfunction of G-CSF is linked to Kostmann Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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