Thermo Fisher Scientific G-CSF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific G-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine G-CSF. Anti-Murine G-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mG-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine G-CSF (500-P69) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of recombinant mG-CSF.

Western Blot:
To detect mG-CSF by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for recombinant mG-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P69BT-1MG will be provided as 2 × 500 µg.

Target Information

G-CSF (Granulocyte Colony Stimulating Factor) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues, and a soluble isoform of the G-CSF receptor has been described.

This pleiotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts, and bone marrow cells. G-CSF has specific effects on the proliferation, differentiation, and activation of hematopoietic cells. It is also expressed by various transformed cells such as carcinoma and myeloblastic leukemia cells.

G-CSF is encoded by two distinct DNA sequences, resulting in a full-size, high-activity and a shorter, low-activity isoform. It is highly conserved among species and exerts its biological functions through interaction with its receptor expressed on hematopoietic progenitors, neutrophilic granulocytes, and certain carcinoma cell lines.

Clinical relevance:
G-CSF is used therapeutically for neonatal infections, acute myocardial infarction, granulocyte transfusion in neutropenia, severe infections and sepsis, chronic autoimmune neutropenia, acute myeloid leukemias, Sweet’s syndrome, and AIDS. Dysfunction of G-CSF has been linked to Kostmann Syndrome and serves as a marker protein for various carcinomas such as bladder cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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