Thermo Fisher Scientific SCF Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific SCF Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

• Western Blot (WB): 0.1–0.2 µg/mL
• ELISA: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine SCF. Anti-Murine SCF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mSCF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SCF (500-P71) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mSCF.

Western Blot:
To detect mSCF by Western blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant mSCF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P71BT-1MG is provided as 2 × 500 µg.

Target Information

Stem Cell Factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF to the c-Kit receptor leads to homodimerization and activation of signaling pathways such as PI-3, PLC-γ, Jak/STAT, and MAP kinase. SCF expression induces mast cell survival and release of histamine, cytokines, and chemokines. Inhibition of the SCF/c-Kit pathway reduces histamine levels, mast cell and eosinophil infiltration, IL-4 production, and airway hyperresponsiveness, suggesting potential therapeutic relevance in inflammatory diseases like asthma. At least two isoforms of SCF are known.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.