Thermo Fisher Scientific B7-H7 Monoclonal Antibody (MA57YW), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg/test)

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MA57YW

Immunogen

B7-H7 recombinant protein

Conjugate

APC APC APC

View additional formats

• PE-Cyanine7
• PE-eFluor 610
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784652

Product Specific Information

Description: This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.

Applications Reported: This MA57YW antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MA57YW antibody has been pre-diluted and tested by flow cytometric analysis of the HDLM2 cell line. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Target Information

B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), whereas other members of the B7 family generally have only two Ig domains (IgV-IgC). B7-H7 is somewhat more closely related to B7-H3 and B7-H4 and shares 10% to 18% amino acid identity and 23% to 33% similarity to B7 family members. B7-H7 protein is widely expressed in epithelium cells of the gallbladder, breast, kidney, colon, small intestines and placenta. In the immune system, HHLA2 protein is not constitutively expressed on human monocytes/macrophages. B7-H7 is widely expressed in cancers, especially in triple negative breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.