Thermo Fisher Scientific Phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) Monoclonal Antibody (EM-55)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 647
• Request custom conjugation

Product Specific Information

This antibody recognizes phosphorylated intracellular tyrosine 111 of CD3 zeta chain (CD247), a component of the TCR/CD3 complex expressed on T cells.

• Flow cytometry: Intracellular staining
• Western blotting: Non-reducing conditions

Target Information

The CD3z complex is essential for transducing antigen-recognition signals into the cytoplasm of T cells and regulating TCR complex expression. T cell activation through the TCR involves CD3 gamma, delta, epsilon, and zeta subunits, which are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11.
These subunits contain double tyrosine-based motifs mediating association with cytoplasmic signaling molecules. CD3 antigens are expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased T lymphocyte percentages may occur in autoimmune diseases. Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3δ/CD3ε/CD3ζ defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.