Thermo Fisher Scientific Phospho-PRAS40 (Thr246) Recombinant Rabbit Monoclonal Antibody (23GB3855)
Phospho-PRAS40 (Thr246) 표적에 특이적인 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 사용 가능. HEK293F에서 발현된 고순도 항체로 인간, 마우스, 랫트 반응성. PBS/glycerol buffer에 보관하며 -20°C에서 안정적 유지.
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Thermo Fisher Scientific Phospho-PRAS40 (Thr246) Recombinant Rabbit Monoclonal Antibody (Clone: 23GB3855)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB3855 |
| Immunogen | Synthetic peptide of human Phospho-PRAS40 (Thr246) (239–253AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after various cell death signals. AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. It is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), which controls cell growth and cycle, and acts as a negative regulator of autophagy. Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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