Thermo Fisher Scientific CD94 Monoclonal Antibody (HP-3D9), APC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HP-3D9

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854571

Product Specific Information

Description: The HP-3D9 monoclonal antibody reacts with human CD94, a 70-kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is expressed by NK cells, NKT cells, as well as subsets of CD8+ T cells and gamma delta T cells. This molecule is present as a heterodimer with members of the NKG2 family of receptors on the cell surface. CD94 dimerization with NKG2A results in an inhibitory receptor that suppresses NK cell function. In contrast, CD94 association with NKG2C and NKG2E leads to formation of an activating receptor complex that binds DAP12, an adapter protein that contains an ITAM motif. The CD94/NKG2 receptor complex binds the non-classical MHC Class Ib molecule HLA-E and plays important roles in adhesion and NK cell activation.

Crossblocking studies indicate that HP-3D9 and DX22 recognize the same epitope.

Applications Reported: This HP-3D9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HP-3D9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.