Thermo Fisher Scientific TNF alpha Polyclonal Antibody
돼지 TNF alpha 단백질에 특이적인 Rabbit Polyclonal 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. Protein A 정제 및 동결건조 형태로 제공되며, 장기 보관 시 -20°C 이하에서 안정적. 면역반응, 세포사멸, 염증 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:2,000–1:2,500 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNF alpha. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for swine TNF alpha protein. A BLAST analysis suggests cross-reactivity with TNF alpha from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against TNF alpha from baboon based on 90% homology. Cross-reactivity with TNF alpha from other species has not been determined.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice indicate a neuroprotective function, and TNF alpha induces tumor necrosis when injected into tumor-bearing mice. It also plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, and contributes to the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, synergistically with interferon-gamma. It has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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