Thermo Fisher Scientific APIP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:200–1:1,000
• Publications: -

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:25–1:100
• Publications: -

Product Specifications

Product Specific Information

The antibody detects endogenous levels of total APIP protein.

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified from human and mouse and designated Apaf 1 (apoptosis protease-activating factor 1).
Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), leading to Caspase-9 activation. Activated Caspase-9 cleaves and activates Caspase-3, a key protease responsible for proteolytic cleavage of many proteins in apoptosis.

Cho et al. have identified a new Apaf 1 Interacting Protein (APIP), also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 for the Apaf 1 binding site.
APIP is predicted to encode a 204 amino acid protein. An isoform, APIP2, encodes a 242 amino acid protein differing in its N-terminus due to alternative splicing.
APIP transcript is ubiquitously expressed in most adult tissues, with high expression in skeletal muscle, heart, and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.