Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Brilliant UV 615
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine, and functions as a neutralizing antibody. IL-4, also known as BCDF, BCGF-1, and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. It promotes B cell proliferation, differentiation, and Ig isotype switching, and upregulates MHC class II and CD23 expression.

• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Validated for intracellular staining of mouse Th2-polarized splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
  • Recommended usage: ≤0.25 µg per test (100 µL final volume).
  • Cell count: 10⁵–10⁸ cells/test (empirically determined).
  • Antibody titration recommended for optimal performance.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is compatible with cytometers using a 405 nm violet laser. Ensure instrument compatibility before use.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product #SB-4401-42), or
• Brilliant Stain Buffer™ (Product #00-4409-75)
  to reduce non-specific polymer interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin-4 (IL-4) is a pleiotropic cytokine produced by activated T cells, acting as a ligand for the IL-4 receptor. It shares overlapping functions with IL-13 via receptor cross-binding. IL-4 signaling involves STAT6 activation and regulates immune responses. IL-4, IL-3, IL-5, IL-13, and CSF2 genes form a cytokine cluster on chromosome 5q, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures.

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