Thermo Fisher Scientific Phospho-EIF4B (Thr420) Polyclonal Antibody
Phospho-EIF4B (Thr420) 특이적 인식하는 Rabbit Polyclonal Antibody로, 인산화된 eIF4B의 내인성 발현 검출에 적합. Western blot 및 IHC(Paraffin) 검증 완료. 1 mg/mL 농도의 액상형 항체로 연구용으로 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EIF4B (Accession P23588), corresponding to amino acid residues around phosphorylated Thr420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816964 |
Product Specific Information
Antibody detects endogenous levels of eIF4B only when phosphorylated at Thr420.
Target Information
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5′-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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