Thermo Fisher Scientific HLA-DQB1 Polyclonal Antibody

Thermo Fisher Scientific HLA-DQB1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive Control: Raji
• Predicted Reactivity: Human (89%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages).

The beta chain is approximately 26–28 kDa and encoded by 6 exons:

1. Exon 1: Leader peptide
2. Exons 2–3: Extracellular domains
3. Exon 4: Transmembrane domain
4. Exon 5: Cytoplasmic tail

Within the DR molecule, the beta chain contains all polymorphisms specifying peptide-binding specificities. Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4, and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4, and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8, and DRB9.

HLA and MHC antibodies play a significant role in immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.