Thermo Fisher Scientific PKC gamma/SCA14 Polyclonal Antibody
PKC gamma/SCA14 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human PKC gamma/SCA14 (amino acids 151–250) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated by intracellular signal transduction pathways. In humans, at least 12 different PKC isoforms have been identified, differing in structure, tissue distribution, localization, and substrate specificity.
PKC alpha, beta I, beta II, and gamma constitute the conventional PKC family, which are Ca²⁺- and phospholipid-dependent. PKC enzymes phosphorylate a wide range of protein targets and play key roles in cellular signaling. They also act as receptors for phorbol esters, known tumor promoters.
PKC gamma is specifically expressed in the brain and spinal cord, localized to neurons. It is involved in neuronal functions such as long-term potentiation (LTP) and long-term depression (LTD), and may play a role in neuropathic pain development. Mutations in PKC gamma are associated with the neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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