Thermo Fisher Scientific alpha Tubulin Polyclonal Antibody
Western blot, IHC, ICC/IF에 적합한 alpha Tubulin 폴리클로날 항체. 사람, 생쥐, 랫트 등 다양한 종에 반응. 항원 친화 크로마토그래피로 정제된 고순도 항체. 세포 구조 연구 및 미세소관 단백질 분석에 적합.
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- PA585157
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.63 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792304 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (95%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. The alpha-beta tubulin heterodimer is the basic building block of microtubules, which are present in almost all eukaryotic cells. Microtubules serve as structural supports and transport lines within the cell and play a key role in mitosis.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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