Thermo Fisher Scientific Rabbit anti-Chicken IgY (H+L) Secondary Antibody, FITC

Applications

Tested Dilution

Immunohistochemistry (IHC)

1:50 - 1:200

Immunocytochemistry (ICC/IF)

1:50 - 1:200

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

1:50 - 1:200

Product Specifications

Species Reactivity

Chicken

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Biotin/host/Rabbit/)
• [HRP](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/HRP/host/Rabbit/)
• [Rhodamine](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/Rhodamine/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Chicken IgY (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Concentration

1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient

RRID

AB_228384

Target

IgY

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Antibody Specificity: This antibody reacts with the heavy chains on chicken IgY and with the light chains common to most chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins using immunoelectrophoresis. However, these antibodies may cross-react with immunoglobulins from other species.

Restoration and Storage: Store product at 2-8°C until opened. Restore to 1.1 mL with distilled water (1.5 mg/mL after restoration). Centrifuge product if it is not clear upon standing for 1-2 hours at room temperature. To judge clarity, draw the product into a pasteur pipette. Product may be stored for several weeks at 2-8°C as undiluted liquid. After dilution, do not use for more than one day.

To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Fluorophore: Fluorescein-5-isothiocyanate (FITC-isomer 1)

Amax = 492 nm; Emax = 520 nm

Fluorophore/Protein: 8.5 µg/mg; 3.2 moles FITC per mole IgG

Country of Origin: USA

Target Information

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.