Thermo Fisher Scientific BRD4 Polyclonal Antibody
BRD4 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태입니다. 인간 및 마우스 시료에 반응하며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:100–1:500 | Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE tissue sections |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1312 and 1362 of human bromodomain-containing protein 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
BRD4 is a ubiquitously expressed nuclear phosphoprotein and a member of the BET family. It is the human homolog of mouse MCAP and contains two N-terminal bromodomains, a central ET-domain, and several kinase-like motifs. BRD4 is expressed as two isoforms, with the longer variant containing a proline- and glutamine-rich C-terminus. It functions as a chromatin-binding protein induced by growth stimuli and plays roles in cell cycle regulation and chromosomal dynamics during mitosis. BRD4 also serves as a cellular target for the E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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