Thermo Fisher Scientific BRD4 Polyclonal Antibody
BRD4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP에 적합. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태, 4°C 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1312 and 1362 of human bromodomain-containing protein 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
Target Information
BRD4 is a ubiquitously expressed nuclear phosphoprotein and a member of the BET protein family. It is the human homolog of mouse MCAP and contains two N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus.
BRD4 is expressed as two isoforms; the longer variant includes a C-terminal region rich in proline and glutamine. It functions as a chromatin-binding protein induced by growth stimuli and participates in cell cycle regulation through interactions with RFC and SPA-1.
BRD4 also plays a role in chromosomal dynamics during mitosis and serves as a cellular target for the E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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