Thermo Fisher Scientific PIGF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues in Human PIGF.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2719627

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Phosphatidylinositol-glycans(PIGs) aremulti-passtransmembrane proteins that localize to the endoplasmicreticulum. PIGs exhibitvariousfunctions but all are crucial for the biosynthesis of the glycosylphosphatidylinositol (GPI) anchor. Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachmentsignal or the lipid portion of GPI. Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetylglucosamine (GlcNAc) to phosphatidyli nositol (PI). A variety of other PIGs play distinct rolesin GPIsynthesis. PIG-F functions as an auxiliary subunit of ethanolamine phosphate (EtNP) transferases. It associates with PIG-O and isrequired for its expression and stability. Together these two PIGs function as an EtNP transferase and catalyze the transfer of EtNP to the third mannose (Man-3) of GPI. Amutation in the gene encoding PIG-F may result in a block of EtNP addition to Man-3 and lead to an absence of GPI-anchored proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.