Thermo Fisher Scientific Phospho-TRIM28 (Ser824) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of TIF1 beta only when phosphorylated at Ser824.

Target Information

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and functions as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through specific interactions with KAP-1 (KRAB-associated protein 1).

KAP-1 is an 835-amino-acid polypeptide containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance transcriptional repression efficiency. KAP-1 also interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin regions containing HP1α, thereby regulating transcriptional repression. Mutations in KAP-1 that disrupt HP1 binding result in random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.