Thermo Fisher Scientific MADCAM1 Monoclonal Antibody (OTI1A5), TrueMAB
MADCAM1 단백질을 인식하는 Mouse monoclonal antibody(OTI1A5)로 Western blot 및 Immunocytochemistry에 적합. HEK293T에서 발현된 full-length human MADCAM1 항원으로 제작. 동결건조 형태, PBS buffer 및 trehalose 포함. 연구용 전용 시약.
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Applications
Western Blot (WB)
- Tested Dilution: 1:4,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A5 |
| Immunogen | Full length human recombinant protein of human MADCAM1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The MADCAM1 protein is an endothelial cell adhesion molecule that interacts preferentially with leukocyte β7 integrin LPAM-1 (α4β7), L-selectin, and VLA-4 (α4β1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues.
It belongs to the immunoglobulin family and is related to ICAM1 and VCAM1.
Multiple alternatively spliced transcripts encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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