Thermo Fisher Scientific AKT Monoclonal Antibody (14E5.16C8.25F6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (IHC)

20 µg/mL

-

ELISA (ELISA)

1:20,000

-

Immunoprecipitation (IP)

1:500-1:3,000

-

Product Specifications

Species Reactivity

Chimpanzee, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

14E5.16C8.25F6

Immunogen

Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.