Thermo Fisher Scientific AKT Monoclonal Antibody (14E5.16C8.25F6)
AKT 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체로, WB, IHC, ELISA, IP 등에 사용 가능. 인간, 생쥐, 랫드, 침팬지에 반응하며 Protein A로 정제된 액상 형태. 세포 성장, 생존 및 대사 연구에 활용.
- 카탈로그번호
- 200-301-401x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific AKT Monoclonal Antibody (14E5.16C8.25F6)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (IHC) | 20 µg/mL |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chimpanzee, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14E5.16C8.25F6 |
| Immunogen | Synthetic peptide corresponding to residues internal to the human AKT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human and mouse AKT protein. BLAST analysis suggests cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat, and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined by Western Blot. Cross-reactivity of AKT from other sources has not been determined.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase that plays an important role in diverse biological responses such as metabolism regulation, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors.
Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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