Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12)
Phospho-ATM (Ser1981) 단일클론 항체로 인간, 마우스, 랫트 시료에 반응. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 단백질 A 정제, 액상형, 고농도(1 mg/mL) 제품으로 DNA 손상 반응 연구에 적합.
- 카탈로그번호
- 200-301-400x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12)
Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 5 µg/mL |
| ELISA | 1:20,000–1:100,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H11.E12 |
| Immunogen | Synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S (aa 1974–1988 of human ATM) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.
By ELISA, reactivity against SLAFEEGSpQSTTISS at 1:1600 dilution shows absorbance >3.000, whereas non-phosphorylated SLAFEEGSQSTTISS shows absorbance of 0.145. Reactivity is observed against human ATM. Cross-reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family. The ATM gene encodes a protein involved in DNA double-strand break repair and cell cycle regulation. ATM phosphorylates multiple downstream targets such as P53, Mdm2, BRCA1, and SMC1. Activation occurs through autophosphorylation upon exposure to ionizing radiation, triggering kinase cascades that regulate cell cycle, apoptosis, and DNA damage repair. ATM dysfunction is linked to hypersensitivity to radiation, impaired DNA synthesis inhibition, and delayed p53 induction, contributing to ataxia-telangiectasia pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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