Thermo Fisher Scientific Phospho-Stat5 (Ser694) Monoclonal Antibody (5F6.F1)
STAT5a 단백질의 Y694 인산화 형태를 특이적으로 인식하는 단클론 항체. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 액상 형태로 제공되며 -20°C에서 안정적 보관 가능.
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- 200-301-B18
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- pk
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Thermo Fisher Scientific Phospho-Stat5 (Ser694) Monoclonal Antibody (5F6.F1)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC(P)) | 20–40 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F6.F1 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein (Phospho STAT5 pY694). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This antibody is specific for mouse Stat5a protein phosphorylated at Y694.
BLAST analysis suggests cross-reactivity with Stat5a from human, mouse, and rat based on 100% homology with the immunizing sequence.
Cross-reactivity with Stat5a from other sources has not been determined.
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. Upon cytokine or growth factor stimulation, STAT proteins are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus and act as transcription activators.
STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones.
Activation of STAT5 alpha in myeloma and lymphoma associated with TEL/JAK2 fusion is independent of cell stimulus and essential for tumorigenesis.
In mouse, STAT5 alpha induces BCL2L1/BCL-X(L) expression, suggesting anti-apoptotic function.
STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues. Differential mRNA accumulation has been observed in muscle and mammary tissue.
STAT5 alpha plays critical roles in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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