Thermo Fisher Scientific Phospho-Stat5 (Ser694) Monoclonal Antibody (5F6.F1)

Thermo Fisher Scientific Phospho-Stat5 (Ser694) Monoclonal Antibody (5F6.F1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is specific for mouse Stat5a protein phosphorylated at Y694.
BLAST analysis suggests cross-reactivity with Stat5a from human, mouse, and rat based on 100% homology with the immunizing sequence.
Cross-reactivity with Stat5a from other sources has not been determined.

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. Upon cytokine or growth factor stimulation, STAT proteins are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus and act as transcription activators.

STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and various growth hormones.
Activation of STAT5 alpha in myeloma and lymphoma associated with TEL/JAK2 fusion is independent of cell stimulus and essential for tumorigenesis.
In mouse, STAT5 alpha induces BCL2L1/BCL-X(L) expression, suggesting anti-apoptotic function.
STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues. Differential mRNA accumulation has been observed in muscle and mammary tissue.
STAT5 alpha plays critical roles in reproduction, lactation, immune function, and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.