Thermo Fisher Scientific Phospho-AKT (Ser473) Monoclonal Antibody (17F6.B11)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Atto 594
• Biotin
• DyLight 488
• DyLight 549
• DyLight 649
• Custom conjugation available upon request

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human and mouse AKT protein phosphorylated at S473. BLAST analysis suggests cross-reactivity with AKT pS473 from human, mouse, rat, and chimpanzee based on 100% sequence homology. Cross-reactivity with AKT2 and AKT3 has not been determined.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. It is activated by insulin, PI3K, IGF1, and other growth factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.