Thermo Fisher Scientific eIF5B Polyclonal Antibody
Rabbit polyclonal antibody recognizing human, mouse, and rat eIF5B. Validated for WB, ICC/IF, and ELISA. Supplied as liquid form at 0.84 mg/mL in PBS with 50% glycerol. Suitable for cytoplasmic protein detection in research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–273 of human EIF5B (NP_0569883) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805974 |
Product Specific Information
Immunogen sequence:
MGKKQKNKSE DSTKDDIDLD ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK QDFDEDDILK ELEELSLEAQ GIKADRETVA VKPTENNEEE FTSKDKKKKG QKGKKQSFDD NDSEELEDKD SKSKKTAKPK VEMYSGSDDD DDFNKLPKKA KGKAQKSNKK WDGSEEDEDN SKKIKERSRI NSSGESGDES DEFLQSRKGQ KKNQKNKPGP NIESGNEDDD ASFKIKTVAQ KKAEKKERER KKRDEEKAKL RKLKEKEELE TGKKDQSKQK ESQ
Positive Samples: DU145, HeLa, Jurkat, LO2
Cellular Location: Cytoplasm
Target Information
Accurate initiation of translation in eukaryotes requires multiple factors, including eIF5B, the eukaryotic homolog of prokaryotic IF2. eIF5B interacts with eIF1A and other initiation factors on the ribosome to position the initiator methionine tRNA on the start codon, ensuring accurate translation initiation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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