Thermo Fisher Scientific HDAC9 Polyclonal Antibody
HDAC9 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. Human에 반응하며, Chicken과 Mouse에도 예측 반응. 고순도 정제 및 안정적 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:1,000 | - |
| Immunoprecipitation (IP) | 1:100 | - |
| ChIP assay (ChIP) | 1–3 µL | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 2–32 of human HDAC9 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion, Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2117071 |
Product Specific Information
This antibody is predicted to react with chicken and mouse based on sequence homology.
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. Remodeling of chromatin is a critical component of transcriptional regulation, and acetylation of nucleosomal histones is a major mechanism of this remodeling. Acetylation of lysine residues in the amino-terminal tail domain of histones induces conformational changes that increase DNA accessibility to transcription factors.
Histone acetylation is mediated by nuclear histone acetylases such as GCN5, PCAF, p300/CBP, HAT1, and the TFIID subunit TAF II p250. Conversely, deacetylation of histones is associated with transcriptional silencing and is mediated by histone deacetylases (HDACs), including HDAC1–9.
HDAC9 and HDAC9a are alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter but retains the catalytic domain and deacetylase activity. Both isoforms repress myocyte enhancer-binding factor 2-mediated transcription and are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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