Thermo Fisher Scientific DHFR Polyclonal Antibody
DHFR 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. IHC 및 ICC/IF에 적합하며, 내부 영역 합성 펩타이드로 면역생성됨. 액상 형태로 제공되며, 단기 4°C 보관 및 장기 -20°C 보관 가능.
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Thermo Fisher Scientific DHFR Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to internal region of human DHFR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2549851 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast carcinoma.
Target Information
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate and is required for the synthesis of purines, thymidylic acid, and certain amino acids.
The functional dihydrofolate reductase gene is mapped to chromosome 5, and multiple intronless processed pseudogenes or DHFR-like genes have been identified on separate chromosomes.
Deficiency of dihydrofolate reductase has been linked to megaloblastic anemia.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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