Thermo Fisher Scientific PRKCE Monoclonal Antibody (OTI2E9), TrueMAB
PRKCE (PKC epsilon) 단백질을 인식하는 Mouse Monoclonal Antibody. Western blot, ICC/IF, Flow cytometry에 적합. Lyophilized 형태로 제공되며 높은 순도의 affinity chromatography 정제 제품. 연구용으로만 사용 가능.
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E9 |
| Immunogen | Full length human recombinant protein of human PRKCE produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve approximately 1 mg/mL antibody concentration.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated by intracellular signaling pathways. In humans, at least 12 PKC isoforms exist, differing in structure, tissue distribution, localization, and substrate specificity.
Conventional PKC isoforms (α, βI, βII, γ) are Ca²⁺- and phospholipid-dependent. PKC family members phosphorylate diverse protein targets and participate in various signaling pathways. They also act as receptors for phorbol esters, known tumor promoters. Each PKC isoform has unique expression and cellular roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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