Thermo Fisher Scientific PRKCE Monoclonal Antibody (OTI4B3), TrueMAB
PRKCE 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. Dog와 Human 시료 반응성이 있으며, 동결건조 형태로 제공됩니다. 고순도의 Affinity chromatography 정제 제품입니다.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B3 |
| Immunogen | Full length human recombinant PRKCE protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882) to ensure carrier-free conditions.
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated by intracellular signal transduction pathways.
In humans, at least 12 PKC isoforms have been identified, differing in structure, tissue distribution, subcellular localization, and substrate specificity.
PKC family members are involved in diverse cellular signaling pathways and serve as receptors for phorbol esters, known tumor promoters.
Each PKC isoform exhibits unique expression patterns and functional roles within cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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