Thermo Fisher Scientific V5 Tag Monoclonal Antibody, AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

Conjugate

AP AP AP

Form

Liquid

Concentration

1-2 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556566

Product Specific Information

NOTE: R962-25 was previously sold under product #46-0287. When re-ordering, please use #R962-25.

R962-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk.

In Western blot applications, no cross-reactivity has been observed in bacterial lysates.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.