Thermo Fisher Scientific V5 Tag Monoclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 106 publications 106 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 8 publications 8 publications

Immunoprecipitation (IP)

-

View 11 publications 11 publications

ChIP assay (ChIP)

-

View 3 publications 3 publications

Affinity Purification (AP)

-

View 1 publication 1 publication

GST Pull Down (GPD)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Immunogen

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

Conjugate

HRP HRP HRP

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556565

Product Specific Information

NOTE: R961-25 was previously sold under product #46-0708. When re-ordering, please use #R961-25.

R961-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

This antibody is functionally tested against 500 ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 10 minute exposure. This antibody has also been tested in Western blot against 50 ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM).

Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.