Thermo Fisher Scientific BrdU Monoclonal Antibody (BU-1), DyLight 488

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Chemical

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BU-1

Immunogen

5-iodouridine covalently coupled to ovalbumin

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537629

Product Specific Information

Unconjugated MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications. DyLight 488 conjugated MA3-071-D488 has been successfully used in flow cytometry applications.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.