Thermo Fisher Scientific Vinculin (Marker of Age-related Macular Degeneration) Monoclonal Antibody (VCL/2575)
Vinculin 단백질을 표적하는 단클론 항체로, 세포 접착 및 세포골격 연구에 활용 가능. Western blot, IHC 등 다양한 응용에 적합. 인간 및 생쥐 반응성. Protein A/G 정제, 액상 형태로 4°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VCL/2575 |
| Immunogen | Recombinant fragment (around aa 174–322) of human Vinculin (VCL) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques: the beta subunit of integrin binds to talin, talin binds to vinculin, which interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element linking the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 7414-MSM4-P0_Vinculin_P18206-1_House_mouse.svg, 7414-MSM4-P0_Vinculin_P18206-1_House_mouse_PDP.jpeg)
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