Thermo Fisher Scientific NCOA2/SRC2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 1–4 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat

Target Information

SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator 2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors. These receptors act as conditional transcription factors, regulating genes involved in cell growth, development, and homeostasis.
Members of the nuclear hormone receptor superfamily include five steroid receptors and class II nuclear receptors, characterized by three domains:

1. N-terminal transcriptional activation domain
2. Central DNA-binding domain
3. C-terminal hormone-binding domain

Before hormone binding, steroid receptors remain inactive in complexes with heat-shock protein-90 and other stress proteins. Hormone binding induces conformational changes, causing dissociation from inhibitory complexes and enabling homodimer binding to specific DNA enhancer elements, thereby modulating gene transcription. Transcriptional coactivator proteins, such as SRC2, are required for transcription initiation after enhancer binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.