Thermo Fisher Scientific CDK4 Monoclonal Antibody (DCS-31)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 10 publications 10 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rabbit, Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DCS-31

Immunogen

Purified recombinant cdk4 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536376

Product Specific Information

This antibody reacts with an epitope at aa 16-35.

Recommended positive controls include MAD109, LS174T, and PC12 cells.

Target Information

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.