Thermo Fisher Scientific Shiga toxin 2A Monoclonal Antibody (11E10)

Thermo Fisher Scientific Shiga toxin 2A Monoclonal Antibody (11E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Product supplied at 1 mL (100 µg/mL).
• Functional Assay: For inhibition of biological activity in vitro, dilutions should be made according to the amount of toxin to be inactivated.
• ELISA: Antibody can be used as detector.

Target Information

Shiga-like toxins (SLTs), also known as Verotoxins, are produced by enterohemorrhagic Escherichia coli (EHEC) strains such as O157:H7, O26:H11, and O111:H8. These strains are associated with hemorrhagic colitis and hemolytic-uremic syndrome (HUS), characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia.

There are several distinct E. coli SLTs:

• SLT-I and SLT-II are produced by EHEC.
• SLT-I shares 99% deduced amino acid sequence homology with Shiga toxin.
• SLT-I and SLT-II share approximately 60% sequence homology and are antigenically distinct.
• Antibodies to SLT-II can also neutralize a variant, SLT-IIv (SLT-Iiv), produced by E. coli strains causing edema.
  SLT-IIv is cytotoxic for Vero cells but not HeLa cells, distinguishing it from SLT-II.

The toxin structure consists of two domains:

• A polypeptide: inhibits protein synthesis by targeting ribosomes.
• B polypeptide pentamer: binds to the eukaryotic cell receptor globotriaosylceramide (Gb3), leading to receptor-mediated endocytosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.