Thermo Fisher Scientific EEF2 Polyclonal Antibody
EEF2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin)에 적합합니다. Human, Mouse, Rat에 반응하며 항원 친화 크로마토그래피로 정제되었습니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 220–500 of Human EEF2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.86 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545138 |
Product Specific Information
- Recommended positive controls: 293T, A431, Neuro2A, NIH-3T3, Neuro 2A, Raw264.7, PC-12
- Predicted reactivity: Mouse (98%), Rat (98%), Chicken (95%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EEF2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this process, the ribosome transitions from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. It facilitates the coordinated movement of tRNA molecules, mRNA, and conformational changes in the ribosome.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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