Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB와 IHC(P)에서 사용 가능. 인간, 마우스, 랫트에 반응하며, 막 및 세포 접합 부위 검출에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태로 안정적인 연구용 시약.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from 550–565 amino acids of human ZO-1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: A431, HEK293
- Cellular location: Membrane, Cellular junction
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins can associate with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the tight junction.
ZO-1 expression is significantly reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions. Unlike ZO-1, which is also expressed at cardiac myocyte junctions, ZO-2 is not found in nonepithelial tissue.
ZO-1 is located on the cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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