Thermo Fisher Scientific Heat Shock 27kDa Protein 1 Monoclonal Antibody (CPTC-HSPB1-2)
Heat Shock 27kDa Protein 1(HSPB1/HSP27)에 특이적인 mouse monoclonal antibody. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 어플리케이션에 사용 가능. 단백질 A/G 정제, PBS buffer, preservative-free. 연구용으로만 사용.
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- 3315-MSM3-P1ABX
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CPTC-HSPB1-2 |
| Immunogen | Recombinant human full-length HSP27 (UniProt ID: P04792-1, antigen range 1–205) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- IHC (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
- Antigen Retrieval: For formalin-fixed tissues, heat sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool to room temperature for 20 minutes.
Target Information
Heat shock proteins (HSPs) are produced by cells in response to stress.
HSPB1 (also known as HSP27) is a small heat shock protein acting as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization.
HSP27 regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in HSPB1 can lead to Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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