Thermo Fisher Scientific CD3/CD25 Antibody Cocktail, FITC, PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

M2AB, ITYV

Immunogen

human thymocytes and PHA-activated human T-lymphocytes

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_1071974

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1) and PE-conjugated CD25 (clone ITYV, isotype IgG1).

Target Information

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets. The Cluster of Differentiation (CD) nomenclature was established to standardize the naming of NK cells. To date, a total of 151 CD clusters and subclusters have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.