Thermo Fisher Scientific CD3/CD28 Antibody Cocktail, FITC, PE

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 10 µL / 1×10⁶ cells

Product Specifications

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1) and PE-conjugated CD28 (clone B-23, isotype IgG1).

Target Information

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon, and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, mediated at least in part by a double tyrosine-based motif present in the CD3 subunits.

CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.
Human CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily, constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than immature cells, and activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen-presenting cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.