Thermo Fisher Scientific CD3/CD28 Antibody Cocktail, FITC, PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

M2AB, B-23

Immunogen

human thymocytes and HPB-ALL T-cell line

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1071988

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1) and PE-conjugated CD28 (clone B-23, isotype IgG1).

Target Information

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.