Thermo Fisher Scientific RIP1 Polyclonal Antibody
RIP1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 인간 시료에 반응하며, 비결합(Unconjugated) 상태로 제공됩니다. 염증 및 세포사멸 신호전달 연구에 유용한 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to amino acids 269–287 of human RIP1 was used as immunogen (GenBank no. AAC32232.1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2718767 |
Target Information
RIP1 is a serine-threonine kinase that mediates inflammatory and programmed necrosis signaling following activation of death receptors, PRRs, and DNA damage. Upon TNFR1 activation by TNF-alpha cytokines, TRADD and TRAF2 are recruited to the receptor. RIP1 phosphorylates DAB2IP at Ser-728 in a TNF-alpha-dependent manner, activating the MAP3K5-JNK apoptotic cascade.
Ubiquitination by TRAF2 via Lys-63-linked chains enhances communication with downstream signal transducers in the MAPK and NF-kappa-B pathways, leading to activation of inflammatory genes. Polyubiquitinated RIP1 binds to IKBKG/NEMO, a key step for NF-kappa-B activation. Interaction with other RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association forms a necrosis-inducing complex.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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