Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech
Rat VEGF-165 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, ELISA, Neutralization 실험에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체이며, -20°C에서 보관. 연구용으로만 사용.
- 카탈로그번호
- 500-P275-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
| Neutralization | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat VEGF 165 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930194 |
Product Specific Information
AA Sequence of recombinant protein:
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat VEGF 165. Anti-Rat VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Rat VEGF 165 matrix.
Sandwich ELISA:
To detect Rat VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat VEGF 165 (500-P275Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Rat VEGF 165.
Western Blot:
To detect Rat VEGF 165 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat VEGF 165 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system are fundamental regulators in angiogenesis signaling. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine linked to this process. Five isoforms of VEGF are generated by alternative splicing from a single VEGF gene. These isoforms bind to tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), expressed mainly on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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