Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
ERK1/2의 Thr202, Tyr204 인산화 상태를 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용 가능. 인간, 생쥐, 랫드 등 반응. 고특이성 및 λ-phosphatase 처리로 검출 신호 소멸 확인 가능.
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Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 13 publications |
| Immunohistochemistry (IHC) | 1:500 | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Plant, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1+2 (p44/42 MAP kinase) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533283 |
Product Specific Information
- Predicted to react with Xenopus and zebrafish based on 100% sequence homology.
- Specific for ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204.
- Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
- Immunolabeling is completely eliminated by lambda-phosphatase treatment.
Target Information
ERK1 and ERK2 are widely expressed kinases involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Activation occurs through multiple stimuli such as growth factors, cytokines, viral infection, and G protein-coupled receptor ligands.
Upon activation, Ras interacts with Raf, leading to phosphorylation of MEK1/2, which subsequently phosphorylates ERK1/2.
Activated ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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