Thermo Fisher Scientific MLLT10 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total AF10.

Target Information

Translocations affecting chromosome 11q23 involve many partner chromosome regions and occur in various leukemias. The 11q23 gene involved in the translocations is MLL. MLLT10 is the partner gene to MLL1 involved in t(10;11)(p12;q23) translocations.

In leukemia patients, the t(10;11)(p12;q23) translocation fuses MLL1, a SET domain-containing histone methyltransferase, to the MLLT10 gene. The MLLT10 gene encodes a 1,027-amino acid protein with an N-terminal zinc finger and a C-terminal leucine zipper domain.

MLLT10 is one of the few MLL partner genes independently rearranged with a third gene, CALM, in the t(10;11)(p12;q14) translocation. Chimeric fusion proteins MLL/AF10 and CALM/AF10 retain the leucine zipper motif of MLLT10, which interacts with GAS41, a protein amplified in glioblastoma. GAS41 interacts with INI1, a component of the SWI/SNF complex involved in chromatin remodeling and transcription regulation. Retention of the leucine zipper suggests these chimeric proteins may interfere with normal gene regulation via chromatin remodeling complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.