Thermo Fisher Scientific HNMT Recombinant Rabbit Monoclonal Antibody (034)
Human HNMT 단백질을 인식하는 recombinant rabbit monoclonal antibody로, 높은 특이성과 민감도를 제공합니다. WB, ELISA, IP에 적합하며, 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 특징으로 합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 0.5–2 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 034 |
| Immunogen | Recombinant Human HNMT protein (Met1–Ala292) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785211 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
This antibody has specificity for Human HNMT.
Target Information
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase (HNMT) and oxidative deamination via diamine oxidase.
HNMT is a cytosolic enzyme that uses S-adenosyl-L-methionine as the methyl donor.
In the mammalian brain, histamine neurotransmitter activity is regulated by N(tau)-methylation, as diamine oxidase is not present in the central nervous system.
A common genetic polymorphism affects the activity levels of this gene product in red blood cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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